Expression of class II Chitinase gene in chilli (Capsicum annuum L.) as response to Fusarium oxysporum pathogen attack
Penulis/Author
Rejeki Siti Ferniah (1); Prof. Rina Sri Kasiamdari, S.Si., Ph.D. (2); Prof. Ir. Achmadi Priyatmojo, M.Sc., Ph.D. (3); Prof. Dr. Budi S. Daryono, M.Agr.Sc. (4)
Tanggal/Date
2 2015
Kata Kunci/Keyword
Abstrak/Abstract
Chilli plants have many mechanism defenses to pathogen attack. The biotic stress can induce genes encoding Pathogenesis Related (PR) proteins to increase expression. The aim of this research is to analyze a molecular responds of CaChi2 gene as PR protein in chilli, which is infected by fungal pathogenic Fusarium oxysporum. Quantitative real-time poly merase chain reaction (qRT- PCR) was used to measure the fluorescence and the ddCt method was used to analyze the expression level of CaChi2 in chilli. The result showed, up-regul ated expression of resistant plants but no change in expression of susceptible plants after many range of time. It is concluded that the CaChi2 gene can be a molecular marker of resistance to Fusarium oxysporum in chilli.
Key words: Chilli, chitinase, Fusarium oxysporum ,qRT-PCR, resistance
Rumpun Ilmu
Biologi (dan Bioteknologi Umum)
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
142-147 Expression of Class II Chitinase Gene in Chilli (Capsicum annuum.pdf
[PAK] Full Dokumen
2
Cek Similarity_Expression of class II Chitinase gene in chilli (Capsicum annuum L_) as response to Fusarium oxysporum pathogen attack.pdf
[PAK] Cek Similarity
3
Full_2015_Expression of Class II Chitinase Gene in Chilli (Capsicum annuum___PAK Rina SK.pdf
