Exfoliative vaginal cytology and vaginal acidity profile in Ettawa-Saanen grade does
Penulis/Author
PRADITA IUSTITIA S (1); Putri Kusuma Astuti, S.Pt., M.Sc. (2); Prof. Dr. Ir. Budi Prasetyo Widyobroto, DESS., DEA., IPU., ASEAN Eng. (3); Dr. Ir. Sigit Bintara, M.Si., IPU., ASEAN Eng. (4); Prof. Ir. Diah Tri Widayati, MP., Ph.D., IPM (5)
Tanggal/Date
4 2018
Kata Kunci/Keyword
Abstrak/Abstract
The knowledge of the reproductive physiology of estrus cycle is important for animal management. The stage of the estrus cycle was predicted through the morphologic, endocrine and secretary changes occurring in the ovaries and the tubular genitalia during the estrus cycle, which had been associated with the level of steroid sex hormone. Vaginal smear is a simple technique to determine the stages of estrus cycle and it is a useful tool in determining op- timum standing heat in does. The aim of this study was to determine the proportion of exfoliative vaginal cell dur- ing the various stages of estrus cycle using vaginal smear techniques in Ettawa-Saanen grade does. Thirteen healthy Ettawa-Saanen grade does with average weigh 35-40 kg, age of 3-4 years and had period of estrus 16-18 days in lac- tating period were used. All does were in natural estrus cycle without synchronization. A vaginal smear and vagi- nal pH were collected from swabbed vaginal epithelium in does. Smear was stained with 3% Giemsa and observed microscopically. Progesterone level of the collected blood serum from vena jugular was assayed by enzyme linked im- munosorbent assay (ELISA). All data were collected on day 0, 3, 12 and 15 of estrus. Period of estrus was predicted from the last three periods of the earlier recording of es- trus cycles. The result showed the proportion of vaginal cell was significant different in each phase of estrus cy- cle. Proportion of parabasal, intermediate, and superfi- cial epithelium cell in the particular period of estrus cycle were 0; 8.2375 ± 6.1301; 91.7624 ±6.1302 in estrus phase, 2.29±6.87; 38.56 ±31.39 ; 59.14 ± 34.37 in met-estrus phase, 83.22±17.66; 15.08±14.91 ; 1.70 ±3.75 in diestrus phase, 9.56±18.13; 70.57± 31.86; 19.87±26.77 proestrus phase re- spectively. The vaginal pH showed in estrus phase were 7.17 ± 0.54, in met-estrus were 6.17 0.30, in diestrus phase were 5.79±0.43, and in proestrus phase were 5.92±0.4.74. Progesterone levels showed in estrus phase were 0.0830.15, in metestrus phase were 0.084±0.11, in diestrus phase were 0.23±0.11 and in proestrus phase 0.23±0.11. In conclusion dominant proportion of superficial cell, a high vaginal pH value and the lowest level of progesterone that occurred in estrus period might be used as the base for determining optimal time for insemination.
