| Abstrak/Abstract |
Introduction: The material for exposed pulp treatment is calcium hydroxide (Ca(OH)2), although there are still some
disadvantages. Composites with components of gelatin, chitosan, carbonated hydroxyapatite, and Tetraethyl orthosilicate
(TEOS) were used to develop the material to obtain optimal quality. Nestin is a marker for differentiation of odontoblast and expressed in irritated odontoblasts. This study evaluated Nestin expression in the dental pulp after applying gelatin-chitosan-TEOS-Ca(OH)2 composite material. Materials and methods: There were 30 Wistar rats divided into two groups, namely the treatment and control groups, with 15 rats in each group. The maxillary first molar tooth was opened using diamond bur No. 010 to the depth of the pulp. The gelatin-chitosan-TEOS-Ca(OH)2 composite was applied in the treatment group, while the control group used Ca(OH)2, followed by temporary fillings. The expression of Nestin was evaluated after 1,3,5,7, and 14 days of application using immunohistochemistry method under light microscopy with 64x and 160x magnifications. Three replication was taken for each evaluation time. Results: A positive expression seen in both the treatment and control groups. The pulp area beneath the irritated tissue showed positive expression of Nestin, especially in the odontoblasts and their processes, pulp cells near positive of the odontoblasts, and flat cells attached to the dentin. The treated group showed weaker Nestin expression than the control group. Conclusion: The gelatin-chitosan-TEOS-Ca(OH)2 composite material has potential as an exposed pulp medicament. After applying the gelatin-chitosan-TEOS-Ca(OH)2
composite to the exposed pulp, the positive Nestin expression indicates a pulp tissue regeneration process. |
