| Penulis/Author |
Prof. Dr. Ir. Aziz Purwantoro, M.Sc. (1) ; TANTRI SWANDARI (2); Dr. Dyah Weny Respatie, S.P., M.Si. (3); Widhi Dyah Sawitri, S.Si., M.Agr., Ph.D. (4); Prof. Dr. Rudi Hari Murti, S.P., M.P. (5) |
| Abstrak/Abstract |
In this study, phenotypic characters and biochemical analysis observed from leaves were carried out to evaluate the
T1 population of transgenic cosmos that had inserted SoSPS1 (Sucrose Phosphate Synthase isoform in sugarcane) by floral
dip using A. tumefaciens strain GV3101 carrying the recombinant plasmid pRI101AN-SoSPS1 and nptII gene as a selection
marker. Sucrose phosphate synthase plays an essential role in sugar accumulation. Furthermore, the gene insertion is
prospectively predicted to influence plant growth characteristics and accumulation of biochemical compounds. Information
related to the impact of SoSPS1 overexpression on the accumulation of biochemical compounds in yellow cosmos is still
relatively rare. The research material is cosmos seeds that have been transformed in previous research. Phenotypic
characters according to UPOV descriptors, confirmation of the presence of the SoSPS1 gene, and biochemical analysis
(reduction sugar, total sugar, sucrose, total phenol, and chlorophyll a, and b) were observed in the T1 plant population. The
results showed the segregation of growth type, stem anthocyanin, branch density, and an increase in the character of
internode length (24.64%) and diameter of the stem (12.21%). The chlorophyll content of leaves of transgenic and wild-type
plants showed similar levels: increased phenol content (17.52%), reduced sugar (28.77%), and total sugar (14.71%). The
higher phenol content in the transgene cosmos can make this plant a suitable resource for possible production of
bioherbicide. |
