| Abstrak/Abstract |
Bubble tea drinks containing tea and tapioca pearl are currently very popular. Streptococcus mutans ATCC 25175 is known as caries-causing bacteria. Metabolism of S. mutans ATCC 25175 by producing glucosyltransferase (GTF). The GTF enzyme has the function of converting sucrose into fructose and glucan which plays a role in the process of biofilm formation. This study aims to evaluate of bubble tea drink on the metabolism S. mutans ATCC 25175.
The study was experimental method and the subjects was S. mutans ATCC 25175 from the Integrated Research Laboratory of Faculty of Dentistry Universitas Gadjah Mada. The test materials
were divided into 4 groups, consisting of bubble tea drinks no sugar, no sugar and bubble, sugar no bubble, sugar and bubble. The study was carried out consist of the biomass index, biostability, changes in pH, the activity of the GTF enzyme and fructose release by S. mutans ATCC 25175. Microtiter Plate Assay was measured using an ELISA reader at wavelengths of 560 nm and 590 nm.
Statistical results using ANOVA and LSD or Dunnett T3 showed the bubble effect could reduce the biomass index, biostability and pH after interacting with S. mutans ATCC 25175 (p<0.05). The bubbles in the bubble tea drink were also able to activate the GTF activity of S. mutans ATCC 25175 after 24 hours of incubation and reduce the fructose enzyme by S. mutans ATCC 25175 after 72 hours of incubation (p<0.05). It was concluded that the metabolism of S. mutans ATCC 25175 was inhibited after interacting with bubble tea drinks |
