| Abstrak/Abstract |
For more than four decades, combination chemotherapy (co-chemotherapy) has been
employed as a means to increase the effectiveness of chemotherapy regiments. The aim of our
research is to investigate the activity of Moringa oleifera L. (tanaman kelor) ethanolic extract
(MEE) as a co-chemotherapy agent with 5-fluorouracil (5-FU) on WiDr colon cancer cell line.
Evaluation of MEE potency as a co-chemotherapy agent with 5-FU was based on cytotoxic
activity based on percent cell viability via MTT assay, and based on apoptosis observation via
the double staining method using acrydin orange – ethidium bromide (AE) as the staining
reagent.Cytotoxicity evaluation of single treatment using concentrations of 5, 20, 50, 100,125,
and 250 ?g/ml of MEE reduced cell viability 24 hours post-treatment. 5, 50, and 250 ?g/ml of
MEE was chosen as the combination concentrations with 1000 ?M 5-FU. MTT assay 24 hours
and 48 hours post-combination treatment showed significant cell viability reduction in
comparison to those of single treatments. Apoptosis observation using the double staining
method shows the presence of apoptotic cells 48 hours post combination treatment. MEE is a
potential co-chemotherapy agent by increasing the sensitivity of WiDr colon cancer cell line
towards 5-FU. |
