| Abstrak/Abstract |
Microorganisms have a high potential as biofuel sources. Co-culture of microalgae and yeasts can result in high lipid productionas a modification treatment. The goal of this study was to see how the co-culture of the Glagah consortium (diversity of associ-ated microalgae and bacteria from Glagah Lagoon, Yogyakarta) and Lipomyces starkeyiaffected the production of biomass, lipids, proteins, and carbohydrates. The culture was performed under airtight conditions on a shaker at 127 rpm, with a lightintensity of 27.75 mol/m2/s and a temperature of 30°C. The culture was subjected to a dark: light (6:18) treatment. Biomass was measured by dry weight, lipids by the Bligh and Dyer method, proteins by the Bradford method and carbohydrates by the phe-nol-sulfuric acid method. On day 3, L. starkeyculture produced the most biomass, yielding 2.21 g/L with a productivity of 0.49 g/L/day. On day 4, the highest lipids produced from co-culture treatment yielded 1.03 g/g with a productivity of 0.21 g/L/day. Thehighest protein yield was obtained from L. starkeyiculture treatment on day 4, yielding 0.60 g/g with a productivity of 0.12 g/L/day. On day 6, co-culture produced the total carbohydrates, yielding 4.78 g/g with a productivity of 0.68 g/L/day. The co-culture treatment produced the highest lipids and carbohydrates production (1.03 g/g and 4.78 g/g) and productivity (0.21 g/L/day and0.68 g/L/day), while L. starkeyiculture produced the highest total biomass and protein production (2.21 g/L and 0.6 g/g) and productivity (0.49 g\L\day and 0.12 g/L/day). In microalgae culture, CO2 generally given directly through the aeration process. In this study, the source of CO2 was yeast, whereas yeast also obtained O2 from microalgae in the consortium for their metabolic process. This mutualism symbiosis will help in providing benefits in reducing the costs for the cultivation process, especially in optimizing the production of biomass an lipids |
