EKSTRAK ETANOLIK TEMU KUNCI (Boesenbergia pandurata) MENGHAMBAT ALDEHYDE DEHYDROGENASE PADA SEL KANKER PAYUDARA 4T1
Penulis/Author
Prof. Dr.rer.nat. apt. Adam Hermawan, S.Farm., M.Sc. (6)
Tanggal/Date
2017
Kata Kunci/Keyword
Abstrak/Abstract
Chemotherapies resistance is a major cause of cancer relapse and triggered by the presence of cancer stem cells (CSCs) leading to high mortality rates in cancer patients. Differentiation of CSCs is regulated by aldehyde dehydrogenase (ALDH) enzyme. The aim of this study is to examine the potency of ethanolic extract of Boesenbergia pandurata (EEBP) to inhibit ALDH activities as a marker of CSCs. The rhizomes of Boesenbergia pandurata were extracted in ethanol by maceration for 54 hours. After filtration, the residue was repeated once by the same procedure and the solvent was evaporated. Phytochemical study of EEBP was investigated by thin layer chromatography (TLC) (n-hexane:ethyl acetate (4:1)) and sprayed with sitroboric afterwards. Cytotoxic effect of EEBP on 4T1 breast cancer cells was determined by MTT assay, EEBP effect on ALDH activity examined with ALDH activity assay, and interaction between cardamonin as the major compound of EEBP and ALDH was determined by molecular docking. Concentrated extract of EEBP were obtained with yield of 7.8%. The TLC results revealed that EEBP contained chalcone group compound. EEBP exhibited cytotoxicity on 4T1 cells (IC50 of 23 ?g/mL). In addition, EEBP inhibited activity of ALDH enzyme at concentration of 5 ?g/mL. Furthermore, molecular docking results showed weak interaction between cardamonin and ALDH, in comparison with native ligand of ALDH. Nevertheless, cardamonin showed potency to compete with native ligand due to similar interacted amino acid residues to ALDH. EEBP were potentially to be developed as cancer stem cell- targeted agents through inhibition of ALDH enzyme activity.
