Efficacy of Quercetin against Polymicrobial Biofilm on Catheters
Penulis/Author
HASYRUL HAMZAH (1); Prof. Dr.rer.nat. apt. Triana Hertiani, S.Si., M.Si. (2); Dr. Sylvia Utami Tunjung Pratiwi, S.Si., M.Si. (3); Prof. dr. Titik Nuryastuti, M.Si., Ph.D., Sp.MK(K). (4)
Tanggal/Date
2020
Kata Kunci/Keyword
Abstrak/Abstract
Every year, the catheter-associated urinary tract infections (CAUTIs) experience a very significant number of
increases. Urinary tract infections constitute about 30% of nosocomial infections, and about 75% of all bacterial
species show biofilm production, which provides survival benefits to offering protection from environmental
stresses and causing decreased susceptibility to antimicrobial agents. Until now the discovery of catheter
antibiofilm compounds is still minimal, therefore the development of a new candidate antibiofilm for
polymicrobial biofilms in catheters is a challenge that must be overcome in preventing catheter-associated
urinary tract infections (CAUTIs). This study aimed to determine the effectiveness of quercetin in inhibited and
decreased polymicrobial biofilm formation in catheters: Staphylococcus aureus, Pseudomonas aeruginosa,
Escherichia coli, Candida albicans. The test for inhibition of the degradation of polymicrobial biofilms on
catheters was determined using the microtiter broth method. The use of quercetin on polymicrobial biofilms was
analyzed by calculating the minimum biofilm inhibitory concentration (MBIC50) and the minimum value of
biofilm eradication (MBEC50). The relationship of quercetin work against S. aureus, P. aeruginosa, E. coli, and
C. albicans polymicrobial biofilms was tested by using scanning electron microscopy (SEM). Quercetin 1%
gave 50% inhibitory activity to the formation of polymicrobial catheter biofilms at 24 h and 48 h at 53.55±0.01
and 50.38 ± 0.01 in comparison to control (nystatin and chloramphenicol). The results also proved evidence of
quercetin activity that can degrade polymicrobial catheter biofilms by 46.48%±0.01 and damage the
polymicrobial biofilm matrix extracellular polymeric substance (EPS) on the catheter. Quercetin seems to
inhibitory activity against the formation of polymicrobial biofilms in catheters and is very potential to be
developed as a candidate for new antibiofilm drugs against urinary tract infections.
Rumpun Ilmu
Biologi Farmasi
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
38_RJPT_13_11_2020.pdf
Bukti Published
2
E_Msg_13_11.pdf
[PAK] Daftar Isi
3
RJPT - Editorial Board.pdf
[PAK] Halaman Editorial
4
RJPT.pdf
[PAK] Halaman Cover
5
Efficacy of Quercetin against Polymicrobial Biofilm on Catheters.pdf
[PAK] Cek Similarity
6
Efficacy of Quercetin against Polymicrobial Biofilm on Catheters.pdf
