Effects of quercetin on the nicotine-induced oxidative status in male Wistar rats: study on c-reactive protein (CRP) and malondialdehyde (MDA) concentrations
Penulis/Author
Deri Riskiyanti Tallo Manafe (1); Prof. Dr. dr. Denny Agustiningsih, M.Kes., AIFM. (2); Dr. Dra. Prasetyastuti, Apt., M.Kes. (3)
Tanggal/Date
2016
Kata Kunci/Keyword
Abstrak/Abstract
Nicotine can cause atherosclerosis by activating nuclear factor-kB (NF-kB) pathway
lead to induce proinflammatory cytokines release as C-reactive protein (CRP) main
regulators. The increase of CRP can induce reactive oxygen species (ROS) and increase
of malondialdehyde (MDA). Quercetin has been proven to have antiinflammatory and
antioxidant effects. This study was conducted to evaluate effect of quercetin on serum
CRP and MDA concentrations in rats induced by nicotine. This was a true experimental
study with post test only control group design. Thirty six of male Wistar rats were divided
into six groups. Group I as normal control received 1 mL/kg BW of NaCl 0.9% solution.
Group II as negative control received 2 mg/kg BW of nicotine and Group III as positive
control received 2 mg/kg BW of nicotine and atorvastatin at dose of 5 mg/kg BW. Group
IV-VI as treatment groups received 2 mg/kg BW of nicotine and quercetin at dose of
25; 50 or 100 mg/kg BW, respectively. Nicotine was given subcutaneously whereas
atorvastatin and quercetin were given orally once per day for 28 days, consecutively.
Serum CRP and MDA concentrations were measured using Rat hs-CRP ELISA kit and
TBARS assay kit, respectively. Data were analyzed using analysis of variance (ANOVA)
continued using LSD post-hoc test. The results showed that quercetin reduced serum CRP
and MDA concentrations in dose dependent manner. Serum CRP concentration on Group
V (173.39 ± 34.85 ng/mL) and Group VI (114.15 ± 43.62 ng/mL) were significantly
lower than that Group II (244.77 ± 37.95 ng/mL) (p<0.05). Furthermore, serum MDA
concentration on Group IV (5.95 ± 0.11 mmol/mL), Group V (3.93 ± 0.09 mmol/mL)
and Group VI (2.14 ± 0.09 mmol/mL) were significantly lower than that Group II (7.29 ±
0.06 mmol/mL) (p<0.05). In conclusion, quercetin reduces the nicotine-induced oxidative
status in rats.
Rumpun Ilmu
Ilmu Kedokteran Dasar & Biomedis
Bahasa Asli/Original Language
English
Level
Nasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Sertifikat Akreditasi_Berkala Ilmu Kedokteran (Journal of the Medical Science).pdf
