| Penulis/Author |
Dr. drg. Sri Suparwitri, S.U., Sp.Ort.(K). (1); drg. Raden Roro Paramita Noviasari, Sp.Ort. (2); Dr. drg. Cendrawasih Andusyana Farmasyanti, M.Kes.Sp.Ort(K). (3); Dr. drg. Ananto Ali Alhasyimi, MD.Sc., Sp.Ort (4) |
| Abstrak/Abstract |
Introduction: Dentofacial harmony can be improved with orthodontic treatment. As people age, there is
an increasing need for orthodontic treatment; this is true for both adults and children. This study aimed to
determine how the activity of transforming growth factor beta-1 (TGF-β1) differs between young and
old rats and what happens to the TGF-β1 level following honey intake. Materials and Methods: Twelve
Sprague-Dawley rats (n=12) were randomly divided into four groups: young rats without honey
administration, young rats with honey administration (YH), old rats without honey administration, and
old rats with honey administration (OH). To move the teeth distally, orthodontic brackets are inserted on
the maxillary incisors with an open coil spring positioned between the teeth. The honey (YH and OH)
groups received honey at a dose of 0.4 mL/200 g body weight daily for 14 days, and gingival crevicular
fluid samples were collected on days 0, 1, 7, and 14 for all groups. Enzyme-linked immunoassay was
used to assess TGF-β1 levels. Results: The results of a three-way analysis of variance and Tukey’s posthoc tests revealed that the TGF-β1 levels in young rats on days 0, 1, 3, 7, and 14 were noticeably higher
than those in older rats (P < 0.05). Young and old rats that received honey had considerably greater
TGF-β1 levels than the control rats (P < 0.05). Conclusion: The results indicate that the TGF-β1 levels
in young rats were much greater than those in older rats, and honey could increase the TGF-β1 levels in
old rats during active orthodontic tooth movement. |
