| Abstrak/Abstract |
Background Curcumin is an active substance isolated from Curcumalonga L. Empirically C. longa L has been consumed during folliculogenesis to prevent pregnancy. The growth and development of granulosa cells depend on presence of FSH, LH, PGF2?, estrogen and estrogen receptor ? (ER?). This study aims to determine effect of curcumin on estrogen secretion and ER? expression in FSH-, LH-, and PGF2?-stimulated pig granulosa cells of medium follicles. Methods The study was an experimental laboratory study with post test-only control group design. Granulosa cells were isolated from medium follicles of pig ovaries and sub cultured in culture media. The study was performed using 16 treatment groups which divided into 4 different stimulation groups (un-stimulated, FSH-stimulated, LHstimulated, and PGF2 stimulated granulosa cells). Each stimulated group was then assign into 4 treatment groups i.e. untreated group and curcumin treated groups of 3 different concentrations. The estrogen level and ER? expression were analyzed by enzymeimmunoassay. Results Estrogen level and ER? expression were not significantly different between curcumin treated and control group (p>0,05). Estrogen level and ER? expression in FSH- or LHstimulated granulosa cells treated by lower concentrations of curcumin was significantly lower than in those untreated (p<0,05). Estrogen level in PGF2?stimulated granulosa cells treated by curcumin were significantly higher than in those untreated (p<0,05), and no significant differences were observed in the ER? expression between PGF2?-stimulated granulosa cells treated by curcumin and those untreated (p>0,05). Conclusions Curcumin is able to decrease expression of ER? in FSH- or LH-stimulated granulosa cells of pig medium follicles. Therefore, curcumin may disturb folliculogenesis and a potential agent for antifertility. |
