| Abstrak/Abstract |
One of the most commonly utilized materials for dental implants is
carbonate hydroxyapatite (CHA). However, its usage must be free of tissue
toxicity. Therefore, this study aimed to investigate how the use of CHA as a dental
implant material affected the development of zebrafish (Danio rerio) embryos .
CHA treatment was administered to 3–3.5 hours to 72 hours post-fertilization
(hpf) embryos. Alterations in embryo and larva shape were all studied to
determine toxicity. Several abnormalities that might arise during development
were also examined by monitoring morphological alterations in embryos and
larvae. Furthermore, heart morphology in larvae aged 72 hpf; heart rate in
embryos aged 24, 48, and 72 hpf; and cardiac histology structure in larvae aged
30 dpf were all used to study heart development. Alizarin Red and Alcian Blue
(ARAB) staining was employed to determine the anatomy of the cranial cartilage
in larvae aged 6 dpf. Data were statistical analysis with SPSS ver. 21 and the
significance was determined using one-way analysis of variance (ANOVA). The
morphological examination results revealed that the embryo and larvae had no
morphological abnormalities. Furthermore, the heart was developing normally ,
according to examinations of morphology, histology, and heart rate. The cranial
cartilage had no flaws, the structure was complete, and the length and angle of
the cranial cartilage did not change between the control and CHA treatments.
Overall, CHA exposure did not affect the development of zebrafish (Danio rerio)
embryos, according to the findings.
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