| Abstrak/Abstract |
The existence of Orchid Mycorrhizal Fungi (OMF) has a role to stimulate growth and support
the supply of orchid nutrition as a biofertilizer agent. This study aimed to determine the association
of mycorrhizal with Phalaenopsis amabilis (L.) Blume which was carried out through the
effectiveness test of two Indonesian orchid mycorrhizal isolates i.e. Ceratorhiza and Trichoderma.
Study Design: This study consisted of 4 treatments. Each treatment was repeated 3 times, each
repetition of 5 plantlets, so that the total plantlet used was 60.
Place and Duration of Study: Laboratory of Plant Biotechnology, Department of Biology,
Universitas Gadjah Mada, Indonesia, between June 2017 and April 2018.
Methodology: The method of inoculating orchid mycorrhizal by placing a plantlet in a petri dish
containing orchid mycorrhizal for 1, 2, 3, and 4 days. Then plantlets are grown on sterile moss growing media and acclimatized in a greenhouse. Observation of each treatment is carried out
every day for the next month. Observation variables include the number of initial and final roots, the
number of live and dead roots, and the number of living and dead plants.
Results: The results of the orchid mycorrhizal induction test showed that the Ceratorhiza inoculation
treatment showed a fluctuation in the mean increase in the number of final roots, live roots, dead
roots, and dead plantlets that were higher than the Trichoderma inoculation treatment. The results
also showed that the best inoculation time on Ceratorhiza and Trichoderma was day 3 and 4. The
adaptation process had the effect of increasing the number of dead roots in weeks 1 and 2. The
adaptation process stopped at the beginning of week 4 with the number of new roots appearing a
lot.
Conclusion: Orchid mycorrhizal Ceratorhiza shows the value of effectiveness test compared with
Trichoderma. The results of this study are expected to be basic information in efforts to cultivate
natural orchids in Indonesia. |
