| Abstrak/Abstract |
Objectives: The aims of this study were to investigate the effect of crude protein and energy level in feed on insulin-like growth factor-I
( IG F -1 ) ex p re s si o n le v el i n a h y br i d ch i c ke n (f e m al e L ay e r c h i cken×male Pelung chicken). Methodology: To study the correlation between
feed type and IGF-1 expression level. Day-old chick (DOC) hybrids were divided into four groups and each group comprised 10 individuals.
First group was fed with feed A [22% Crude Protein (CP) and 3100 kcal kgG1 Metabolizable Energy (ME)], second group was fed with feed
B (20% CP and 3300 kcal kgG1 ME), third group was fed with feed C (21% CP and 2800 kcal kgG1 ME) and fourth group was fed with
standard feed after starving for 3 days post hatching. Chickens were observed from post-hatch to the 7th day and weighed (at 0, 3 and
7 days) and five individuals were sacrificed at the 7th day for IGF-1 analysis. Total RNA was isolated from the liver for gene expression
analysis. Real-time PCR was used to study the IGF-1 expression profiles of each group after being normalized with $-actin. Results: The
highest body weight was recorded for group 1, followed by group 2, 3 and 4. The IGF-1 expression level was noticeably high for feed A,
B and C groups by 4.82, 3.82 and 1.9 fold, respectively, compared with the starved group. The weight highly correlated with IGF-1
expression level (R2 = 0.8). This moderate growth response to nutrition makes the hybrid chicken a suitable meat-type chicken.
Conclusion: The result suggested that feed containing 22% CP and 3100 kcal kgG1 ME is a promising starter for the Indonesian hybrid
chicken.
Key words:
Effect of Varying Level of Crude Protein and Energy on Insulin-like Growth Factor-I Expression Level in Indonesian Hybrid Chicken (PDF Download Available). Available from: https://www.researchgate.net/publication/312543636_Effect_of_Varying_Level_of_Crude_Protein_and_Energy_on_Insulin-like_Growth_Factor-I_Expression_Level_in_Indonesian_Hybrid_Chicken [accessed May 3, 2017]. |
