| Abstrak/Abstract |
Biodiesel is an alternative energy that can be sourced from oleaginous microbes. The utilization of oleagninous microbes is increasingly being developed along with the need for alternative energy sources in the form of single-cell oil. BR 2.2 isolates are classified as oleaginous organisms because of their ability to accumulate lipids for 28.44% of the total dry biomass. The speed of agitation in the lipid production process is one of the major factors for the effectiveness of the production process and the production costs. This study was conducted to determine the effect of the agitation speed and the incubation time on biomass production and lipid profile of BR 2.2 isolate by submerged fermentation. The stages of biomass and lipid production of BR 2.2 isolate were carried out at agitation speeds of 0 rpm, 50 rpm, 100 rpm, 150 rpm, and 200 rpm. Each incubation time of 0 hours, 48 hours, 96 hours, and 144 hours, the product was harvested in the form of total biomass, lipids, and residual glucose, and nitrogen in the growth medium as measured by DNS and Kjeldahl analysis. Measurement of growth is known based on the increase in the size of the pellet diameter. The highest production yield occurred at 100 rpm with a total biomass of 2.78 g/L and a total lipid of 0.63 g/L for 144 hours of incubation. The uniqueness of the BR 2.2 isolate in lipid production was seen from the percentage of nitrogen consumption which was ≤42.57% of the total nitrogen given in the growth medium. Based on the research conducted, it can be concluded that the agitation speed and incubation time affect the production of total biomass and lipids in the growth medium. |
