| Abstrak/Abstract |
The purpose of this study was to evaluate the
effect of synbiotic products extract of inulin dahlia tuber and
L.casei AP on mice Balb-C strain induced enteropathogen
escherichia coli. The steps of this study were the preparation of
EPEC bacteria cell suspension and synbiotic products;
treatment in an animal, weigh of mice, fecal and pH value; IgA
level by ELISA and SCFA analysis by GC and GC-MS. This
study shows that synbiotic products can reduce diarrhea in
these experimental animals by increasing body weight (24.05
g), feces weight (785.35 mg) and decreased fecal pH (7.08).
Total of the LAB in mice's fecal 9.01 (log CFU/g) was higher
than total Escherichia coli 7.45 (log CFU/g) after treatment by
synbiotic product. IgA levels in the duodenum (1.65 ng/mL)
and ileum (1.45 ng/mL) were higher than jejunum (1.36
ng/mL). SCFA analysis with GC and GC-MS showed that the
difference in a metabolite of treatment group 2 dose of
diarrhea compared to other groups due to butyric acid,
isobutene and 2- methyl propionic. The synbiotic effect is
stimulating microbial shift, IgA, and production of metabolite
optimally at dose 2. |
