Effect of Some Selected Herbal Plant Extracts as Potential Dental Plaque Biofilm Inhibitors
Penulis/Author
Prof. Dr.rer.nat. apt. Triana Hertiani, S.Si., M.Si. (1); Dr. Sylvia Utami Tunjung Pratiwi, S.Si., M.Si. (2)
Tanggal/Date
21 2010
Kata Kunci/Keyword
Abstrak/Abstract
Dental plaque formation is initiated by mouth cavity microbes built biofilm. Plants have been knows as a potential source of antibacterial and biofilm inhibitor compounds. This research aim to screen the potency of several herbal plants extracts as an antiplaque active agent, by in vitro inhibition activity against the planktonic growth and biofilm formation of S. mutans ATCC 21752. Fifteen extracts were obtained by macerating the pulverized-dried samples in petroleum ether for defatting, and the residue was further macerated in 70% ethanol. Antibacterial and inhibiton of biofilm formation were measured using microdilution in polystyrene 96-wells plate at 37°C for 24 h. The percentage of inhibition was calculated by comparing the absorbance os samples to the vehicle (control) measured by plate reader at 595 nm. Samples for biofilm formation inhibition were first stained by 1% crystal violet. The above assays were performed in triplicate, while Listerine® was used as a control. This research revealed that the ethanolic extract of Temulawak and Jahe rhizomes showed potential antibacterial agents and biofilm formation inhibitortowards S. mutans. jahe extract showed MIC90 at 0.091% w/v; MBC at 0.91% w/v; IC50 at 0.040% w/v; and EC50 at 0.013% w/v.
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
International Conference On Medicinal Plants The Future.pdf
