Karya
Judul/Title Effect of screenhouse on disease severity and coat protein diversity of Begomovirus-infected Capsicum frutescens L. ‘Cempluk’ from Indonesia
Penulis/Author Dr. Aprilia Sufi Subiastuti, S.Si. (1); ANGGUN CINDITYA PUTRI (2); CINDY GRESYLLIA PERMADANI (3); Prof. Dr. Budi S. Daryono, M.Agr.Sc. (4)
Tanggal/Date 28 2021
Kata Kunci/Keyword
Abstrak/Abstract Chili can be infected by Begomovirus through whiteflies (Bemisia tabaci) serving as a vector insect. Begomovirus infection causes dwarf plants and yellow curly leaves. The molecular detection of Begomovirus coat protein gene may serve as a preliminary identification of Begomovirus. This study was conducted to observe the differences in the symptom severity of Begomovirus infection in chilis (Capsicum frutescens L. ‘Cempluk’) planted inside and outside a screenhouse. This study also observed whether or not using a screenhouse in chili farming affects the diversity of the coat protein of Begomovirus. Symptom observation and sampling were conducted in Madurejo, Prambanan, Sleman. Molecular detection was performed by amplifying the coat protein gene using the CP Begomovirus universal primer Krusty and Homer. Results showed seven plant samples with DNA bands ± 550 bp and confirmed that the plants were positively infected with Begomovirus. The amplified bands were purified and sequenced. The nucleotide sequences were analyzed using BLASTn, followed by phylogenetic analysis using MEGA. Planting chili in the screenhouse resulted in low disease severity and good crop conditions. The coat protein sequence showed different strains of Begomovirus that infected the chili plants inside and outside the screenhouse. PepYLCIV was found inside the screenhouse while PepYLCIV [Ageratum] was dominant outside the screenhouse. Both strains are closely related to other PepYLCV from various regions in Indonesia. Optical manipulation using an ultraviolet screen or screenhouse was effective in reducing Begomovirus infection and improving plant performance.
Rumpun Ilmu Biologi (dan Bioteknologi Umum)
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
111 JTAS-2031-2020_Aprilia.pdf[PAK] Full Dokumen
2sim-Effect of Screen House on Disease Severity and Coat Protein Diversity of Begomovirus-infected Capsicum frutescens L_ ‘Cempluk’ from Indonesia.pdf[PAK] Cek Similarity