Effect of Kecombrang Flower (Etlingera elatior) Ethanolic Extract on the Number of Macrophages in Periodontitis induced with Hyperglycemia
Penulis/Author
TIFFANY WINATA (1); Prof. Dr. drg. Ahmad Syaify, Sp.Perio(K) (2); drg. Rezmelia Sari, MSc., Sp.Perio (3); drg. Vincensia Maria Karina, MDSc., Sp.Perio. (4); Prof. Dr. drg. Juni Handajani, M.Kes., Ph.D (5)
Tanggal/Date
30 2024
Kata Kunci/Keyword
Abstrak/Abstract
Chronic inflammation can mediate diabetes mellitus and periodontitis and will exacerbate these con- ditions through a bidirectional relationship. Periodontitis-induced inflammation triggers immune cell activation, one of which is macrophages. The study aimed to determine the effect of kecombrang flower (Etlingera elatior) ethanolic extract on macrophage number in periodontitis of Wistar rats induced with hyperglycemia. Methods: A 70% ethano- lic extract of kecombrang flowers was prepared, with flavonoid content identified through Thin Layer Chromatogra- phy (TLC). Thirty-two Wistar rats induced by hyperglycaemia and periodontitis were divided into two groups of equal size, namely treatment and control group. Induction of hyperglycaemia was carried out by intraperitoneal injection of streptozotocin (40 mg/kg BW). Periodontitis was induced using silk ligature with size 3/0 in the subgingival area of the mandibular incisors for 7 days. The treatment group received intraperitoneal injections of the extract (100 mg/ kg BW) while control administered saline, daily for seven days. Gingival tissue was collected on days 1, 3, 5, and 7 post-injections, processed histologically by Haematoxylin-Eosin staining, followed by macrophage counting. Two- way ANOVA and LSD post hoc test (p>0.05) were used to analyze data. Results: Hyperglycaemia and periodontitis were indicated by fasting blood sugar of 369.6 mg/dL and clinical signs. The macrophage counts reached peak on day 3 then decreased gradually on days 5 and 7. The macrophage counts in the saline group were higher than those in the treatment group. Conclusion: Injection of 70% ethanolic extract of kecombrang flower as an anti-inflammatory agent effectively reduces macrophage number in hyperglycemic and periodontitis.
