| Penulis/Author |
Woro Anindito Sri Tunjung, S.Si., M.Sc., Ph.D. (1) ; VITA FATONAH (2); GHEA PUTRI CHRISTY (3); Sugeng Triono, S.Si., M.Si. (4); Lisna Hidayati, S.Si., M.Biotech. (5); DWI PRIYANTO (6); Prof. Dr. Yekti Asih Purwestri, S.Si., M.Si. (7); Aries Bagus Sasongko, S.Si., M.Biotech. (8); HENNISA (9); NUR FAIZAH (10); Dr.rer.nat. Ari Indrianto, S.U. (11) |
| Abstrak/Abstract |
Our previous study showed that kaffir lime leaf extracts may have anticancer properties. However, production of bioactive compounds is affected
by environmental factors. Here, we present a method to control
environmental conditions using in vitro culture techniques such as callus
induction. Calluses were induced from seed embryo explants of kaffir lime on
MS medium with combinations of 2,4-D and BAP at concentrations 1:0.5; 1:1;
and 2:1, respectively. Fourty and 60 days-old calluses were extracted using
chloroform and ethyl acetate and analyzed by GC-MS. Results showed all
combinations of 2,4-D and BAP were able to induce callogenesis from seed
embryo explants of kaffir lime with no significant differences of callus
initiation time, biomass, morphology and growth rates. However differences
were detected in the bioactive compound profiles. In kaffir lime callus, both
fatty acids and secondary metabolites were detected. Specifically, in 40 daysold calluses (exponential growth phase) we detected ?-pinene and 1.8–
cineole in plants treated with 2,4-D: BAP at concentration 1:0.5 and 2:1. In 60
days-old calluses (stationary phase) we detected a number of compounds in
plants treated with 2,4-D:BAP at concentrations of 1:0.5 and 2:1, including
caryophyllene, linoleoyl chloride, thiogeraniol, stigmasterol, clianosterol,
citronellal, neo-isopulegol, citronellol, geraniol, eugenol, cyclopropane,
pristane, elemol and farnesol.
Keywords: 2,4-D (2,4-diclorophenoxyacetic acid); Bioactive compounds; BAP
(Benzyl amino purine); Callus; kaffir lime
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