Effect of Green Tea Extract Gel as an Antioxidant on Macrophage Cell Count after Bleaching on Wistar Rats’
Penulis/Author
Ovy Prima Damara (1); drg. Henytaria Fajrianti (2); Dr. drg. Raphael Tri Endra Untara, M.Kes., Sp.KG(K) (3); Prof. Dr. drg. Juni Handajani, M.Kes., Ph.D (4); Dr. drg. Ema Mulyawati, M.S., Sp.KG(K) (5); Dr. drg. Yulita Kristanti, M.Kes., Sp.KG.(K) (6)
Tanggal/Date
30 2024
Kata Kunci/Keyword
Abstrak/Abstract
Introduction: Hydrogen peroxide for bleaching procedure, can penetrate the interprismatic space of enamel and dentin tubules, leading to pulp irritation. Oxidative stress resulting from this irritation triggers an inflammatory reaction in the pulp, increasing the number of macrophage cells. One approach to mitigate the cytotoxic effects of bleaching agents diffusing is the application of antioxidants. Green tea contains numerous catechins known for their antioxidant activity. This study aims to investigate the impact of applying green tea extract gel (Camellia sinensis) at concentrations of 5% and 10% on macrophage cell count in the tooth pulp of Wistar rats’
after an extra coronal bleaching procedure using 40% hydrogen peroxide. Materials and methods: Fifteen upper molars from Wistar rats’ divided into three groups. The control group underwent the bleaching procedure alone. The teeth were rinsed in the control group with warm distilled water, while the first group received 5% and second group received 10% green tea extract gel. On the 5th day, molar specimens were collected and prepared
for histological examination using hematoxylin-eosin (HE) staining. Macrophage cell counts were calculated using a light microscope at 400x magnification. Results: The Results of one-way ANOVA test revealed a significant influence of green tea extract concentration on macrophage cell count (p < 0.05). LSD post hoc test indicated significant differences in all compared groups. Conclusion: Application of 10% green tea extract concentration resulted in a lower macrophage cell count compared to both the 5% concentration and the control group.
