Effect of Green Tea (Camelia sinensis) Extract Gel Concentration of 5% and 10% on Odontoblast Cells after Extracoronal Bleaching with 40% Hydrogen Peroxide
Penulis/Author
Nina Indrawati Rosita (1); Dr. drg. Yulita Kristanti, M.Kes., Sp.KG.(K) (2); Prof. Dr. drg. Juni Handajani, M.Kes., Ph.D (3); drg. Pribadi Santosa, M.S., Sp.KG. (4); drg. Margareta Rinastiti, M.Kes., Sp.KG. Subsp.KR(K)., Ph.D. (5)
Tanggal/Date
30 2024
Kata Kunci/Keyword
Abstrak/Abstract
Introduction: Hydrogen peroxide, a bleaching agent for teeth, can reach and damage pulp tissue by diffusion through enamel and dentine structures. Pulp inflammation, in the form of reactive oxygen species (ROS), causes discontinuity of the odontoblast layer. Antioxidants are common ROS suppressants, one of which is polyphenols found in green tea (Camelia sinensis). The aim this in vivo study was to determine the effect of 5% and 10% green tea extract gel concentration in odontoblast cells of the Wistar rats’ teeth after extracoronal bleaching with 40% hydrogen peroxide. Materials and methods: Fifteen molar teeth of male Wistar rats were applied with a bleaching agent on the occlusal surface. The teeth in the control group were rinsed with warm distilled water after bleaching.
In the experimental groups, the teeth were applied with 5% and 10% green tea extract gel after bleaching. Wistar rats were sacrificed on the fifth day after treatment, followed by hematoxylin-eosin staining. Histological examinations were observed under light microscope using 400x magnification, followed by determination of discontinuity scoring. Results: The results of Kruskal-Wallis test showed a significant effect of the green tea extract gel concentration for the odontoblast layer discontinuity in all groups (p < 0.05). Mann-Whitney test result showed significant difference in all compared pair groups (p < 0.05). Conclusion: The application of 10% green tea extract gel shows the lowest discontinuity score compared to the 5% extract gel application and the control group.
