| Abstrak/Abstract |
Background: Single bout of downhill running exercise causes muscle injury and
excessive TNF-? pro inflammatory cytokine resulted in impaired of muscle
regeneration trough inhibition of satellite cell proliferation and differentiation. The
ability of omega 3 to decreased inflamation is used to enhance muscle regeneration
after injury.
Objective: To investigate the effect of fish oil omega 3 supplementation on decreased
levels of TNF-?, proliferation and differentiation of satellite cells after muscle injury.
Methods: Male wistar rats were divided into control group (non injury), muscle
injury unsupplemented group and fish oil supplemented group with different doses:
low (3,6 mg/200mgBB); medium (5,4 mg/200mgBB) and high (7,2 mg/200mgBB) at
2 hour after exercise via oral sonde. Subject had performed 90 min downhill running
(5 min running, 2 min rest). Blood sample were taken from retroorbital vein 24 hour
following exercise for TNF-? measurement and solei muscle surgically removed 72
hour following exercise for MyoD and Myogenin expression measurement.
Results: There was significantly decreased in TNF-? levels on fish oil supplemented
groups compare to unsupplemented group. MyoD and Myogenin expression as a
proliferation and differentiation of satellite cell marker at 72 hour following exercise
significantly higher in fish oil supplemented group than unsupplemented group. Fish
oil supplementation with high doses causes delayed cell proliferation contribute to
impaired cell regeneration.
Conclusion: Fish oil omega 3 increases proliferation and differentiation of satellite
cells through MyoD dan Myogenin expression by decreasing TNF-? level in a
dependent doses. |
