Effect of Extract Gel Green Tea (Camelia Sinensis) on Neutrophil of Post Extracoronal Bleaching Rat’s Teeth
Penulis/Author
Dr. drg. Yulita Kristanti, M.Kes., Sp.KG.(K) (1); Prof. Dr. drg. Juni Handajani, M.Kes., Ph.D (2); Dr. drg. Raphael Tri Endra Untara, M.Kes., Sp.KG(K) (3); drg. Pribadi Santosa, M.S., Sp.KG. (4)
Tanggal/Date
31 2024
Kata Kunci/Keyword
Abstrak/Abstract
Background: Free radicals produced by hydrogen peroxide after teeth bleaching which diffuse through the enamel and dentinal tubules can trigger
inflammation of the pulp. To overcome the negative side effect, exogenous antioxidants have been proposed. This research was conducted to ascertain
the impact of natural antioxidants, specifically green tea at 5% and 10% which was applied on rat molars teeth that were bleached using 40% hydrogen
peroxide.
Method: Fifteen molar teeth of male Wistar rats were bleached using 40% hydrogen peroxide on their occlusal surface. Furthermore, the control group
(Group I) rinsed using warm distilled water, while in Group II and Group III, the teeth were rinsed using 5% and 10% green tea extract gel. Wistar rats were
sacrificed on the fifth day after treatment, followed by haematoxylin-eosin staining. Histological examinations were observed under an illumination
microscope using 400x magnification and the number of the neutrophil were counted.
Result: The number of neutrophils was significantly impacted by the concentration of green tea extract gel, according to the results of a one-way
ANOVA test (p 0.05). The findings of the post hoc test indicated that every pair of groups analyzed had significant differences (p<0.05).
Conclusion: Group III which was treated using10% green tea extract gel has the lowest number of neutrophils compared to Group II which was treated using
5% green tea extract gel and the control group.
