Effect of Distinct Nitrate Concentrations on Pigment Content of Mixed Culture of Chlorella vulgaris and Dunaliella sp.
Penulis/Author
RAHAYU DIAN EKA P (1); Ir. Yano Surya Pradana, S.T., M.Eng., IPM., ASEAN Eng., ACPE., APEC Eng. (2); Mochamad Donny Koerniawan (3); Prof. Dr. Lucia Tri Suwanti, drh, MP (4); Dr. Ir. Ulfah Juniarti Siregar M.Agr. (5); Prof. Dr.Eng. Ir. Arief Budiman, M.S., IPU. (6); Dr. Eko Agus Suyono, S.Si., M.App.Sc. (7)
Tanggal/Date
17 2022
Kata Kunci/Keyword
Abstrak/Abstract
Chlorella vulgaris and Dunaliella sp. are prominent microalgae that are generally used for natural
pigment stocks. These species have been established as producer of valuable bio compounds such as
chlorophyll, carotenoids, and astaxanthin. For this research, C. vulgaris and Dunaliella sp. were cultivated in
mixed culture under nitrogen stress treatments. Total four treatments at distinct nitrate concentrations of
0.012, 0.025, 0.5 NaNO3 g/L, respectively including nitrogen starvation was incorporated in this study.
Nitrogen limitation and nitrogen starvation are widely used techniques to manipulate metabolic pathways
into secondary pigments biosynthesis in microalgae cells. In addition, spectrophotometric measurement
was conducted to investigate every single pigment content in all four treatments of distinct nitrogen
concentrations for 14 days. The highest chlorophyll-a (23.65 mg/L), chlorophyll-b (10.02 mg/L), and total
carotenoids (7.61 mg/L) were obtained from mixed culture with a concentration of 0.025 g/L NaNO3 in
the last period cultivation. Conversely, the highest content of astaxanthin (0.6 mg/L) in mixed culture of
C. vulgaris and Dunaliella sp. in the last period cultivation was achieved from starvation of nitrogen as well.
The microalgae demonstrated robust growth in the presence of diverse bacterial community in the culture.
It is well known that microalgae are associated with the microbiota on its phycosphere biofilm. Yet, it is
unclear which specific bacteria taxa have a role in supporting or inhibiting microalgae growth.
Rumpun Ilmu
Biologi (dan Bioteknologi Umum)
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
02-Putri-et-al.pdf
[PAK] Full Dokumen
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Effect of distinct nitrate concentrations on pigment content of mixed culture of Chlorella vulgaris and Dunaliella sp_.pdf
[PAK] Cek Similarity
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7 FINAL Surat Keterangan Publikasi-Rahayu Dian Eka Putri-Effect of Distinct Nitrate Concentrations on Pigment Content of Mixed Culture of Chlorella vulgaris and Dunaliella sp.pdf
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
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7 PAK FINAL-opt-Rahayu Dian Eka Putri-The Effect of nitrate concentrations on pigments.pdf