| Abstrak/Abstract |
Objective: To determine the effect of carbonate apatite (CHA) hydrogel-aPRF on osteoblastogenesis during relapse in rabbits.
Setting and Sample Population: Oryctolagus cuniculus rabbits from a commercial supplier were investigated in a laboratory setting.
Materials and methods: Forty-five rabbits were divided into three groups (n= 15), namely, the control, CHA, and CHA-aPRF groups. An open-coil spring was compressed between brackets to distalize the lower incisors of the rabbits, by deliver a force of 50 cN for 1 week. The new position of the teeth was retained for 14 days, and CHA hydrogel-aPRF was injected every 7 days. The appliances were then debonded to allow relapse. On days 0, 3, 7, 14, and 21 after debonding, TGF-?1 and BMP-2 expression was examined using immunohistochemistry staining and Runx-2 levels were analyzed by enzyme-linked immunosorbent assay (ELISA). The data collected were analyzed using ANOVA and a post hoc Tukey’s test (P < .05).
Results: Histomorphometric analysis revealed that TGF-?1 expression in the CHA-aPRF group is statistically higher than that in the other groups on days 0, 3, and 7 after debonding (P < .05). BMP-2 expression in the CHA-aPRF group was also statistically higher than that in the other groups on days 3, 14, and 21 after debonding (P < .05). ELISA showed that Runx-2 levels are slightly higher in the CHA-aPRF group than in the other groups (P > .05).
Conclusion: Although injection of CHA-aPRF aids in osteoblastogenesis associated with enhancing TGF-?1 and BMP-2 expressions, it does not significantly upregulate Runx-2 levels. |
