| Penulis/Author |
Dr. Ir. Sigit Bintara, M.Si., IPU., ASEAN Eng. (1) ; Ir. Andriyani Astuti, S.Pt., M.Sc., Ph.D., IPM., ASEAN Eng. (2); Ir. Panjono, S.Pt., MP., Ph.D., IPM., ASEAN Eng. (3); Dr . Ir. Tri Anggraeni Kusumastuti, S.P., M.P., IPM (4) |
| Abstrak/Abstract |
Spermatozoa are easily damaged by lipid peroxidation during processing of semen. Therefore to
minimize this damage and to protect spermatozoa from oxidative damage, antioxidants are used
against the lipid peroxidation. Coconut water is characterized by its high contents of antioxidants.
The beneficial effects of Vitamin E as antioxidants on coconut water diluent of semen was
researched. In this study the most active and available form of vitamin E, the α-tocopherol was
used. Semen samples were collected from 12 heads of Fat-Tailed Sheep twice a week for 4 weeks
using artificial vagina. The pooled semen samples were diluted with coconut water and Vitamin
E (0.4 g/100 ml). The control consisted of coconut water diluent without vitamin E. The results
showed that the motility was higher (P<0.05) in coconut water diluent with Vitamin E (50.8 ±
2.9%) compared to the control (46.8 ± 3.2%). Viability was higher (P<0.05) in the coconut water
diluent with vitamin E (55.1 ± 2.8%) compared to the control (50.2 ± 3.3%). Intact plasma
membrane was higher (P<0.05) in the coconut water diluent with vitamin E (44.8 ± 3.8%)
compared to the control (42.8 ± 2.7%). This study concluded that additional Vitamin E as
antioxidants on coconut water diluent can improve the quality of frozen semen i.e. highest values
of motility, viability, and intact plasma membrane. This could make artificial insemination more
successful. Successful artificial insemination is important to keep the valuable genetic of our local
breeds of sheep, namely Fat-Tailed Sheep. |
