| Abstrak/Abstract |
Guava seed is by-product from the consumption of guava fruits. We
interested to explore further the potential of guava seed waste using
fermentation method. The purpose of this research was to determine
the ability of biofilm formation produced from fermentation of guava
seed. Fermented guava seed was prepared by solid-state fermentation
method using banana leaves wrap at 37 °C for 72 h. It was then
continued with isolation and screening of bacteria from the
fermentation products, preparation of bacteria cultures to be used in
biofilm formation, and formation of biofilm by glass slides and broth
cultures methods. The edible biofilm formation by glass slide method
was observed by light microscopy using 0.5 % Crystal Violet dye, while
biofilm formation by broth cultures method was observed by
transmission electron microscopy (TEM) using phosphotungstic acid 2
% dye. The results show that there were 3 (three) strains lactic acid
bacteria (LAB) candidates isolated from fermented guava seed waste
product (J6, J7, and J8 strains). The observation by light microscopy
showed that J7 strain was the only strain which was unable to form
biofilm by glass slide method. All the strains showed the ability to form
biofilms in different stages by broth cultures method. Thus, guava seed
fermentation was able to produce edible biofilm but the LAB strains still
need to be identified further.
|
