| Penulis/Author |
DIANA JULAIDY PATTY (1); Dr.Sc. Ari Dwi Nugraheni, S.Si., M.Si. (2); Prof. drg. Ika Dewi Ana, M.Kes., Ph.D. (3); Prof. Dr. Eng. Yusril Yusuf, S.Si., M.Si., M.Eng. (4) |
| Abstrak/Abstract |
This study aims to design a 3D carbonate-hydroxyapatite (CHA)/sago (S) based egg white (EW) 13
microstructure with antibacterial properties to improve the performance of bone grafts for bone 14
tissue engineering. In this study, Pinctada maxima (P. maxima) shell was used as a calcium 15
(Ca) source in the CHA synthesis. The annealing temperature of CHA at 900oC, 1000oC, and 16
1100oC affected microstructural and lattice parameters, with stoichiometry 1.72-1.77, and B- 17
type CHA was identified. CHA/S with various concentrations of EW (10 and 30wt%) 18
effectively increased pore size and porosity. XRD spectra confirmed that sago and EW in CHA 19
nanocomposite stable the crystal structure. FTIR spectrum shows protein phosphorylation in 20
CHA nanocomposite due to PO4
3-
ion exchange. In-vitro bioactivity of CHA-S10 (MTT assay) 21
showed increased cell viability and optical density (24h-48 h) to control. Antibacterial activity 22
of CHA-S10 and CHA/S (control) against bacteria associated with periodontal disease and 23
bone infection (Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, 24
Fusobacterium nucleatum [gram negative], and Staphylococcus aureus [gram positive]) by 25
disc diffusion method showed that CHA-S10 and CHA/S had strong antibacterial activity. In 26
conclusion, EW's properties had proven the CHA/S/EW as bone grafts, effectively increasing 27
pore size, porosity, biocompatibility, and strong antibacterial properties. |
