| Abstrak/Abstract |
Ralstonia syzygii subsp. celebesensis, the causal agent of blood disease of banana (BDB),
was first reported in the early 1900s in Selayar Island, South Sulawesi Province, Indonesia. Since the 1980s, R. syzygii subsp. celebesensis has been spreading to most islands in Indonesia, and it was recently reported in Malaysia. R. syzygii subsp. celebesensis causes wilt of the banana plant and rot of fruit. A draft genome assembly of R. syzygii subsp. celebesensis isolate
UGMSS_Db01, isolated from BDB-symptomatic banana in Indonesia, was generated using the Oxford Nanopore Technologies GridION Platform. The genome assembly was 3,568,564 bp,
with 92× genome coverage for the chromosome and 1,614,128 bp, with 102× genome coverage for the plasmid. This draft genome will enrich resources to study the banana–R. syzygii subsp. celebesensis interaction and enhance development of research to control BDB.
Blood disease of banana (BDB), caused by Ralstonia syzygii subsp. celebesensis, has significant impact on banana (Musa spp.) production, and banana is an important food crop in the world. In Indonesia, there is a high diversity of cultivated banana for both fresh and cooking use (Blomme et al. 2017; Ekasari et al. 2012; Li et al. 2013; Ploetz et al. 2007). Symptoms include wilting of the leaves, brown-red vascular staining, and rotten fruit pulp (Blomme et al. 2017; Tjahjono and Eden-Green 1988). BDB was first reported in South Sulawesi in the 1900s and, since the 1980s, the disease has been spreading around the country; recently, it was reported in Malaysia (Ray et al. 2021; Teng et al. 2016; Rijks 1916; Safni et al. 2014). The disease is transmitted by banana-visiting insects, birds, and bats;
contaminated tools; and in other mechanical ways (Buddenhagen 2009). The movement and distribution of R. syzygii subsp. celebesensis-contaminated animals, and human mobil-
ity in transporting infected fruit and suckers for replanting have contributed to long-distance
spread of BDB (Gaumann€ 1921; Safni et al. 2018). R. syzygii subsp. celebesensis is a non-fluorescent Gram-negative bacterium, rod shaped, not spore forming, slow growing, and does not have a capsule structure (Denny and Baek 1991; Ray et al. 2021). A control method for the disease is urgently needed to stop the spread and reduce the yield loss;
however, information about the pathogen is still limited compared with that of the related pathogen, R. solanacearum, which has been widely studied. The first genome of blood dis-
ease bacterium was published in 2017, isolated from Perak, Malaysia as the expansion of
the disease from Indonesia (Badrun et al. 2017; Ray et al. 2021; Teng et al. 2016).The molecular identity of R. syzygii subsp. celebesensis from Indonesia as the origin of the
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