The investiga
tion of talok
frui ts (Mun tingia calabura L.)
was
shown the antioxidant activity of
aqueous fraction of
the
e thanolic extract is rela tively low. Hyd rolysis trea tme nt
has
increa se
d
the antioxidant
activity by re leasing the fla vonoid aglycone from glycoside f
orm. This s tudy aims to de termine the effect of
acid and alkaline hydroly sis, and hydrolysis time on the an tioxidant activity of aqueous fraction of calabura
fruits e thano lic extract. The a ntioxidant ac tivity of acid h ydrolyz ed aqueous fractio
ns in 1 and 3
h hydrolysis,
respective ly 9.5 and 1.5 times more poten t than th e aqueous fraction
, while the a lka line in 1 and 3
h hydro
-
lysis were 2.5 and 6.5 ti mes. Flavonoid aglycone liberated on acid hyd rolysis and alka line had differen t a nti
-
oxidant activity. The v
alue of IC
50
by acid hydrol
yzed aqueous f raction in 1h and 3
h
hydro lysis of 20.55
and 97.88
?g/
mL, while the a lka line in 1h and 3
h h
ydrolysis of 66.64 and 25.53
?g/mL. One hour acid
hydrolysis had antioxidant ac tivity g reate r than 3h wh ereas in alkaline the grea test an
tioxidant activity is
shown in 3
h.
