Double-Coated Nanoparticle of Ribosome Inactivating Protein (RIP) from Mirabilis jalapa L. prepared from Chitosan-Sodium Tripolyphosphate and Alginate-Calcium Chloride: The New Strategy for Protein Drug in Oral Delivery.
Penulis/Author
AMALIA MIRANDA (1); Dr. Hilda Ismail, M.Si., Apt. (2); Dr.rer.nat. Ronny Martien, M.Si. (3); UMMI HADIBA C (4); ARIYANI KUSNIASARI (5); Dewa Ayu Arimurni (6); Made Dwi Pradipta Wahyudi (7); Prof. Dr. apt. Sismindari, S.U. (8)
Tanggal/Date
15 2023
Kata Kunci/Keyword
Abstrak/Abstract
Oral delivery of protein drugs is challenging due to the instability of the compound and structural
barrier exists in the gastrointestinal (GI) tract. Nanoparticle technology is known as a promising drug
delivery strategy to ensure drug bioavailability. This study aims to formulate an oral delivery system of a
potential anticancer agent named Ribosome Inactivating Protein from Mirabilis jalapa L.-C (RIP MJ-C)
through double-coated nanoparticles prepared from Chitosan-Sodium Tripolyphosphate (TPP) and
Alginate-Calcium Chloride (CaCl2). Nanoparticles were prepared through the ionic gelation method, with
the core nanoparticle (RMJCN-1) formulated in the pH of 3.5-5.5 using 0.3-0.5 % w/v of chitosan and 0.03
% w/v TPP. The RMJCN-1 optimum formula was selected to be subsequently coated with the second layer
of alginate and CaCl2, called RMJCN-2, with a concentration of 0.3% w/v and 0.1-0.3 %, respectively. The
sample was characterized by the entrapment efficiency (EE), physical appearance, particle size,
polydispersity index (PI), and potential zetta. The result showed the optimum RMJCN-1 formula with of
EE value of 57.10 ± 0.04 % was obtained by formulating 0.5 % w/v chitosan and 0.3 % w/v STPP in pH
5.5. The optimum RMJCN-2 was obtained by the combination of alginate 0.3 % w/v and CaCl2 0.1% w/v
in the outer layer. This final formula produces nanoparticles with a zeta potential of -14.4 mV, 739.8 nm in
size, with good stability during 7 days at room temperature. This study has successfully developed a
formulation of double-coated nanoparticles from Chitosan-TPP and Alginat-CaCl2 for RIP MJ-C, leads to a
safe nanocarrier system for oral delivery of RIP MJ-C that ensures its bioavailability.
