DNA extraction procedure based on magnetite nanoparticle: A Plackett-Burman design optimization
Penulis/Author
Stanley Evander Emeltan Tjoa (1); Prof. Drs. Mudasir, M.Eng., Ph.D. (2); Prof. Dr.Eng. Edi Suharyadi, S.Si., M.Eng. (3); Prof. Dr. Budi S. Daryono, M.Agr.Sc. (4)
Tanggal/Date
2025
Kata Kunci/Keyword
Abstrak/Abstract
The MNPs-based DNA extraction method is a commonly used method, but research for its optimization has rarely been done using OFAT. Plackett-Burman is an experimental design used to analyze many factors. In this study, we used PlackettBurman to screen several factors in the MNPs-based DNA extraction step such as MNPs type, MNPs amount, MNPs solvent, binding buffer, washing buffer, washing method, drying and elution buffer on the concentration and purity of the extracted DNA. Based on Plackett-Burman analysis, none of the factors had a significant effect on the concentration and purity of the extracted DNA. The analysis also informed the interaction effect between the MNPs amount (in the range 2 – 5 mg) and type (the use of TEOS up to 0.25 w/v) on DNA concentration even though it was not significant. Model 9, one of the models given by the Plackett-Burman matrix was used to extract DNA from environmental bacteria and several types of specimens spiked with Mycobacterium tuberculosis. The extracted DNA has good integrity. It could also be amplified by conventional PCR and probe-based qPCR methods.
Rumpun Ilmu
Biologi (dan Bioteknologi Umum)
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
DNA extraction procedure based on magnetite nanoparticle.pdf
