Difference between Microscopic and PCR Examination Result for Malaria Diagnosis and Treatment Evaluation in Sumba Barat Daya, Indonesia
Penulis/Author
Dwita Anastasia Deo (1); dr. Elizabeth Henny Herningtyas, M.Si., Ph.D. SpPK(K) (2); Dr. dr. Umi Solekhah Intansari, M.Kes., Sp.PK(K) (3); dr. Taufik Mulya Perdana, M.Sc. (4); Prof. dr. E. Elsa Herdiana Murhandarwati, M.Kes., Ph.D (5); Prof. dr. Marsetyawan Heparis Nur Ekandaru, M.Sc., Ph.D. (6)
Tanggal/Date
29 2022
Kata Kunci/Keyword
Abstrak/Abstract
Abstract: Microscopic examination is the backbone of malaria diagnosis and treatment evaluation in
Indonesia. This test has limited ability to detect malaria at low parasite density. Inversely, nested
polymerase chain reaction (PCR) can detect parasites at a density below the microscopic examination’s detection limit. The objective of this study is to compare microscopic and PCR results when
being used to identify malaria in suspected patients and patients who underwent dihydroartemisinin–
piperaquine (DHP) therapy in the last 3–8 weeks with or without symptoms in Sumba Barat Daya,
Nusa Tenggara Timur, Indonesia. Recruitment was conducted between April 2019 and February 2020. Blood samples were then taken for microscopic and PCR examinations. Participants
(n = 409) were divided into three groups: suspected malaria (42.5%), post-DHP therapy with fever
(4.9%), and post-DHP therapy without fever (52.6%). Microscopic examination found five cases of
P. falciparum + P. vivax infection, while PCR found 346 cases. All microscopic examinations turned
negative in the post-DHP-therapy group. Conversely, PCR result from the same group yielded
29 negative results. Overall, our study showed that microscopic examination and PCR generated
different results in detecting Plasmodium species, especially in patients with mixed infection and in
patients who recently underwent DHP therapy
Rumpun Ilmu
Ilmu Kedokteran Tropis
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
tropicalmed-07-00153.pdf
[PAK] Full Dokumen
2
Difference between Microscopic and PCR Examination Result for Malaria Diagnosis and Treatment Evaluation in Sumba Barat Daya, Indonesia.pdf
[PAK] Cek Similarity
3
difference between-komplit_compressed.pdf
[PAK] Full Dokumen
4
BK Tambahan_Difference between Microscopic and PCR Examination Result for Malaria Diagnosis and Treatment Evaluation in Sumba Barat Daya, Indonesia _rev2.pdf
[PAK] Bukti Korespondensi Penulis
5
Difference between Microscopic and PCR_rev_Jun 2023.pdf
