| Abstrak/Abstract |
Begomovirus is one of the most devastating diseases of melon causing high economic losses for farmers. The most ecofriendly strategy to overcome Begomovirus infection is to develop resistant or tolerant cultivar. However, this strategy is
time consuming, expensive and complex, especially for hybrid selection process. A pair of Sequence Characterized
Amplified Region (SCAR) marker was successfully developed from OPA-4 Random Amplified Polymorphisms DNA
(RAPD) marker to discriminate potential Begomovirus resistant genotypes from the susceptible ones. Leaf samples were
amplified using OPA-4 primer and produced specific band at 1198 bp. The band was purified from gel then sequenced. The
sequence was analyzed and extended until 20 bp so it becomes a more specific SCAR marker. BLAST analysis showed that
amplified sequence had 95.8% identity with Cucumis melo L. mitochondrial DNA. To ensure the consistency of marker,
SCAR marker was applied in bulk F2 plants. The results showed that the marker was consistent amplified same DNA
fragment only in Begomovirus-uninfected samples. Chi-square test determined that the F2 bulk segregated following 3:1
pattern for resistant. It was likely indicated that Begomovirus resistance trait was possibly controlled by single dominant
genes. Furthermore, this marker has been validated using eight different melon cultivars and confirmed not to be affected by
environmental conditions.
Keywords: Begomovirus, PCR, Cucumis melo, L, SCAR, RAPD |
