Karya
Judul/Title Development of PCR-RFLP technique for identify several members of Fusarium incarnatum-equiseti species complex and Fusarium fujikuroi species complex
Penulis/Author SYAFIQA PRAMUNADIPTA (1); Prof. Ani Widiastuti, S.P., M.P., Ph.D. (2); Dr. Ir. Arif Wibowo, M.Agr.Sc. (3); Haruhisa Suga (4); Prof. Ir. Achmadi Priyatmojo, M.Sc., Ph.D. (5)
Tanggal/Date 1 2022
Kata Kunci/Keyword
Abstrak/Abstract Fusarium incarnatum-equiseti species complex (FIESC) contain over 40 members. The primer pair Smibo1FM/Semi1RM on the RPB2 partial gene has been reported to be able to identify Fusarium semitectum. Fusarium fujikuroi species complex (FFSC) contain more than 50 members. Fusarium verticillioides as member of this complex can be identified by using VER1/VER2 primer pair on the CaM partial gene. In this research, the Smibo1FM/Semi1RM can amplify Fusarium sulawesiense, F. hainanense, F. bubalinum and F. tanahbumbuense, members of FIESC at 424bp. The VER1/VER2 can amplify Fusarium verticillioides, F. andiyazi, and F. pseudocircinatum, members of FFSC at 578bp. PCR-RFLP by using the combination of three restriction enzymes EcoRV, MspI and HpyAV can differentiate each species of FIESC used. The two restriction enzymes HpaII and NspI can distinguish each species of FFSC used. The proper identification process is required for pathogen control in the field in order to reduce crop yield loss.
Rumpun Ilmu Ilmu Hama dan Penyakit Tanaman
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1Submitted_2021_The Plant Pathology Journal_Development PCR RFLP.pdfBukti Submitted
22021_Syafiqa RFLP_PPJ-NT-12-2021-0184.pdfBukti Published
3Cek Similarity - Development of PCR-RFLP technique for identify several members of Fusarium incarnatum-equiseti species complex and Fusarium fujikuroi species complex.pdf[PAK] Cek Similarity
4Full dokumen - Development of PCR-RFLP technique for identify several members of Fusarium incarnatum-equiseti species complex and Fusarium fujikuroi species complex.pdf[PAK] Full Dokumen
5Surket Publikasi Pak Arif 2.pdfDokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
6Bukti korespondensi-Development of PCR-RFLP technique for identify several members of Fusarium incarnatum-equiseti species complex and Fusarium fujikuroi species complex.pdf[PAK] Bukti Korespondensi Penulis