Development of Automatic Dual Sequence Control Temporary Immersion Bioreactor Systems for Micropropagation Coelogyne pandurata Lindl
Penulis/Author
Anisa Dewi Rahayu (1); LATHIEF AL UMAMI (2); Shiddharta Arya Anggoro Cen (3); BIMO RIZKI A (4); LEONARD SEAN (5); KRESNA BUDIMAN (6); LABRIYANTOKO K (7); Ir. Muhammad Akhsin Muflikhun, S.T., MSME., Ph.D (8); Dr. Ir. I Wayan Mustika, S.T., M.Eng. (9); Prof. Dr. Endang Semiarti, M.S., M.Sc. (10)
Tanggal/Date
2023
Kata Kunci/Keyword
Abstrak/Abstract
Coelogyne pandurata Lindl. is an endemic orchid species of Indonesia from East Kalimantan
and Papua. It is locally known as the “black orchid” due to the distinctive black coloration on its labellum
with intricate green and hairy lines. Unfortunately, the population of this orchid has significantly declined
in its natural habitat, rendering it a rare endangered species. Temporary immersion bioreactor systems have
been proven to be efficient for endemic plant micropropagation with performance enhancements and
innovations. This study aims to develop an optimized bioreactor design and innovate the automatic control
performance of temporary immersion bioreactor systems based on previous research. The control system
developed consists of three modules, namely Graphical User Interface (GUI) module, Sequence Control
(SC) module, and Hardware Interface (HI) module. The GUI module receives information regarding time
and duration of immersion and gas exchange, then the information is synchronized by the SC module, which
plays the role of starting and stopping the processes, while HI module executes the order of the automatic
control system in the immersion and gas exchange process. The developed bioreactor design and control
system offer convenience, require less labor, and ensure precise control over the optimum conditions for
black orchid micropropagation.
