Development of a methodology based on headspace gas chromatography-ion mobility spectrometry for the rapid detection and determination of patin fish oil adulterated with palm oil
Penulis/Author
ANGGITA ROSIANA P (1); Maria Jose Aliaño Gonzalez (2); Marta Ferreiro-González (3); Dr. Widiastuti Setyaningsih, S.T.P., M.Sc. (4); Prof. Dr. Abdul Rohman, S.F., M.Si., Apt. (5); Prof. Dr. apt. Sugeng Riyanto, MS. (6); Miguel Palma (7)
Tanggal/Date
2020
Kata Kunci/Keyword
Abstrak/Abstract
The present research intends to develop a new method based on headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) for the detection and determination of patin fish oil adulterated with different percentages of palm oil, because it is a cheaper vegetable oil. Five variables that affect headspace generation (incubation time and temperature, amount of sample, injection volume, and pre-heating time) have been optimized by means of a Box Behnken design in combination with Response Surface Methodology. Then, repeatability and intermediate precision have been studied where coefficients of variation lower than 10% were obtained. This new method has been applied to several samples of patin fish oil adulterated with palm oil at 20 different levels (5–50% palm oil content). The results have confirmed the suitability of the HS-GC-IMS for a rapid, easy, and reliable detection and discrimination of adulterated oil samples since a characteristic fingerprint that allows 100% successful discrimination between adulterated and unadulterated patin oil samples was achieved. Finally, a regression model has been developed to determine each sample’s adulteration level with an error lower than 10% and a coefficient of determination greater than 0.95.
Rumpun Ilmu
Farmasi Makanan dan Analisis Keamanan Pangan
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
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Development of a methodology based on headspace gas chromatography-ion mobility spectrometry for the rapid detection and determination of patin fish oil adulterated with palm oil (3).pdf
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