| Abstrak/Abstract |
Due to inadequate drug tissue penetration and low blood supply to the bone, the
systemic delivery of medications during infection and inflammation of bone tissues
frequently fails to heal abnormalities or lesions in bone tissues. In the quest for local
delivery of antibiotics to treat the infection and bone-grafting particles to stimulate
bone growth and regeneration, a series of composite films containing gelatin (G),
chitosan (CH), carbonated hydroxyapatite (CHA), and various amounts of tetraethyl
orthosilicate (TEOS) crosslinker were synthesized. The synthesis resulted in 4 (four)
different composite films having a mass ratio of 0.3/0.3/0.5/x, where x=0, 1.87, 3.73,
and 5.60 for G/CH/CHA, G/CH/CHA/TEOS(2), G/CH/CHA/TEOS(4), and G/CH/
CHA/TEOS(6), respectively. The composite films were characterized using SEM for
morphology, SAA for specific surface area and pore volume, and FTIR and XRD
for functional groups and crystal phase, respectively. Furthermore, tensile strength,
water absorption capacity, polymer matrix degradation, Ca2+ release profile, drug
loading capacity, drug unloading (release) profile, and drug release kinetics were
determined to gain insights into the critical design parameters for preparing this
drug carrier. A commercial film, Dentium™ (collagen-based), was included in
the water absorption, drug loading capacity, drug release profile, and degradation
tests. The biological performance of the film was evaluated from protein absorption
and MC3T3I1 cell cytotoxicity. It was found that G/CH/CHA/TEOS(2) exhibited
the lowest total pore volume, the highest tensile strength and protein absorption,
similar water absorption and drug loading capacity, the lowest drug release rate,
the lowest Ca2+ release rate, the lowest degradation rate, and cytocompatibility
when compared to the other synthesized composite films. According to empirical
mathematical modeling, the Higuchi and Korsmeyer-Peppas models best described
the drug unloading (release) process for G/CH/CHA and G/CH/CHA/TEOS films
through a diffusion process. When Dentium™ was included in the tests, Dentium™
exhibited the lowest water absorption, the lowest drug loading capacity, the highest
drug release rate, and the lowest film degradation rate compared to all studied films. |
