Development of a Biobank from a Legacy Collection in Universitas Gadjah Mada, Indonesia: Proposed Approach for Centralized Biobank Development in Low-Resource Institutions
Penulis/Author
Jajah Fachiroh, SP., M.Si., Ph.D. (1); dr. Ery Kus Dwianingsih, Ph.D., Sp.PA(K) (2); Amirah Ellyza Wahdi (3); Linda Tri Pramatasari (4); Sunandar Hariyanto, S.T., M.Eng. (5); Nenes Prastiwi (6); dr. Junaedy Yunus, M.Sc., Ph.D. (7); Maimuna Mendy (8); Bart Scheerder (9); dr. M. Lutfan Lazuardi, M.Kes., Ph.D. (10)
Tanggal/Date
19 2019
Kata Kunci/Keyword
Abstrak/Abstract
Introduction: The establishment of a biobank requires specific expertise along with relatively expensive in-
frastructure and appropriate technology. This causes certain challenges in biobank implementation for research
in low–middle-income countries. Biobank development with established specimens and data collection (legacy
collection) was an approach used in the Faculty of Medicine, Public Health and Nursing, Universitas Gadjah
Mada. This approach aimed to identify the resources available at present, while providing nontechnical in-
formation for further development of a centralized biobank.
Materials and Methods: Retrospective modeling was done in 2015 by recruiting existing specimen collections
and their associated data. The steps were as follows: (1) informing research stakeholders through discussion
with experts and stakeholders; (2) identifying specimen collections to be used; (3) determining the system,
infrastructure, and consumables needed; (4) determining inclusion criteria; (5) building an in-house database
system; (6) organizing data and physical specimen collections; and (7) validating data and physical sample
arrangement. All technical procedures were built into standard operating procedures.
Results: The model included specimens from one -80C freezer. The associated data included demographic,
clinical diagnosis, and physical sample information. Samples came from six studies, collected between 2001
and 2014. A web-based database was built based on the MySQL programming system. Information on bio-
specimens from a total of 4196 subjects collected in 11,358 vials was entered into the database, following
physical rearrangement of vials in the -80C freezer with one-dimensional barcodes taped to vials, boxes, and
racks. A validation test was done for data concordance between the database and physical arrangement in the
-80C freezer, showing no discrepancies.
Conclusion: This report demonstrated current technical and nontechnical insights to further develop a cen-
tralized biobank for health research at an academic institution in Indonesia.
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