Development and validation of a simple HPLC-UV method for the quantification of andrographolide in rabbit plasma
Penulis/Author
YANDI SYUKRI (1); Dr.rer.nat. Ronny Martien, M.Si. (2); Prof. Dr.rer.nat. apt. Raden Rara Endang Lukitaningsih, S.Si., M.Si. (3); Prof. Dr. apt. Agung Endro Nugroho, S.Si., M.Si. (4)
Tanggal/Date
1 2017
Kata Kunci/Keyword
Abstrak/Abstract
In this present work, a simple, rapid and accurate HPLC-UV method has been developed for the quantification of andrographolide in rabbit plasma. The assay was performed using an XTerra® MS C18 column (150 mm X 4.6 mm, 5 ?m) with a mobile phase of methanol and water (60:40), at 0.8 mL/min flow rate and UV detection of 229 nm. Andrographolide was extracted from a biological sample by applying acetonitrile as a precipitating and extraction solvent. The results showed a good linearity with r = 0.9992; the accuracy reported as % diff was found to be -6.42 – 6.55% while the recovery was 99.09, 98.55, and 105.14% for low, medium and high spiked plasma, respectively. The precision (reproducibility) reached 1.08–3.20% RSD for the sample studied. The 2.87% relative standard deviation (RSD) value for selectivity test indicated a good selectivity of the developed method. The developed method is simple and rapid, so that it can be applied for the quantification andrographolide in animal models during pharmacokinetics studies.
Rumpun Ilmu
Analisis Farmasi dan Kimia Medisinal
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
28_ Naskah-IJDDT,Vol7,Issue1,Article3.pdf
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Daftar isi-Volume7,Issue1 - IJDDT.pdf
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3
Editorial Board IJDDT.pdf
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4
Halaman sampul IJDDT Journal Home.pdf
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Development and validation of a simple HPLC-UV method for the quantification of andrographolide in rabbit plasma.pdf
