| Abstrak/Abstract |
Pigmented rice become popular consumed by the public as a functional food. However, there are factors limiting pigmented rice production, namely bacterial leaf blight caused by Xanthomonas oryzae pv. Oryzae (Xoo). The use of resistant varieties that have the Xa resistance gene is considered effective in overcoming the problem of decreasing rice yields. This Xa gene consists of several genes, one of which is a Xa21 gene. This study aims to determine the presence of the Xa21 bacterial leaf blight resistance gene in black rice cultivars Sembada Hitam, Cempo Ireng, Melik and Hitam Toraja and red rice cultivars of Aek sibondang, Merah Sumbawa, Segreng, and Pari Eja in Indonesia. The research methods included isolating the rice genome, checking the results of DNA isolation with agarose gel electro-foresis (0.8%), measuring the concentration and purity of DNA, amplification of DNA using specific primers Xa21, and data analysis. The results of this study detected the presence of Xa21 gene in all cultivars of black and red rice with resistant properties. |
