| Abstrak/Abstract |
Trypanosomiasis (Surra) is a protozoa disease in animals caused by Trypanosoma evansi that is infected mechanically by biting flies. Surra caused economic disadvantages in livestock, particularly horses, cattles, and buffalos in Indonesia. In addition, Surra is immunosupresive and potential to be zoonotic disease. This study was conducted to detect Trypanosoma evance molecularly using Loop-mediated Isothermal Amplification (LAMP) Test. The method was sensitive and specific diagnostic assay capable of detecting T. evansi. There were three isolate DNA samples of T. evansi in Indonesia (Brebes, Lampung and Bengkulu). Amplification was possible when a water bath was used to maintain the temperature at isothermal conditions (60-65 °C), and results could be read by visual observation of colour change. The LAMP test was efficient and robust, and results were obtained within 1 hour. These findings have increased the prospects for developing a simple molecular test that can be used with limited equipment at point of care in endemic rural areas. |
