| Abstrak/Abstract |
Background and Aim: Surra in domestic cats is caused by Trypanosoma brucei and Trypanosoma evansi. However,
molecular diagnostic approaches are required owing to similarities in their morphology. In Yogyakarta, a domestic cat was
diagnosed with trypanosomiasis; however, the causative species was undetermined. Therefore, we aimed to molecularly
and biologically identify the isolate.
Materials and Methods: Ten mice were inoculated intraperitoneally with trypanosomatid to determine their biological
characteristics, and the parasitemia pattern and viability of the animals were observed. Internal transcribed spacer (ITS)-1
amplification was used to assess the molecular characteristics of trypanosomatid.
Results: The prepatent period of this trypanosomatid is between 2 and 4 dpi, whereas the life span of mice is approximately
4–10 dpi. Morphologically, the trypomastigote in the cat blood smear had long slender and intermediate shapes. However,
only the long slender form was detected. Among the total of 410 nucleotides (NT) of ITS-1 sequences, 25 NT substitutions
differed between the cat and mouse isolates. Phylogenetic analysis revealed that both samples had a close genetic relationship
with T. evansi. |
