| Abstrak/Abstract |
Strawberries (Fragaria spp.) represent a fruit with high economic value. However, its cultivation
in Indonesia has not been optimized. Along with the development of technology, plant researchers
and breeders have been developing new cultivars to improve the quality of strawberry plants.
Morphological selection of superior quality cultivars is found less effective, leading to the
development of molecular research. One method of breeding strawberry plants is to use the
Cleavage Amplified Polymorphic Sequence (CAPS) markers which can be used for the
identification of octoploid strawberry cultivars. The purpose of the present study was to investigate
the polymorphic banding pattern of strawberry cultivars grown in Indonesia, to determine CAPS
marker capabilities for detection of polymorphisms, and to determine the degree of similarity of
strawberry cultivars in Indonesia based on the CAPS markers. Samples were 13 strawberry
cultivars from the Citrus and Subtropical Fruit Research Institute of Malang and two strawberry
cultivars from the Center for Strawberry Agro-tourism in Desa Banyuroto of Magelang. DNA
amplification was performed using the primers APX4 and PYDB, and the products of DNA
amplification were cut using the enzymes HaeII and TaqI. Dendrogram construction was analyzed
using the Unweight Pair-Group Using Arithmetic Average (UPGMA) clustering by means of the
software Multi-Variate Statistical Average (MVSP). Results showed polymorphic DNA patterns
from the two CAPS primer used in 15 strawberry cultivars. The phylogenetic relationships of the
15 strawberry cultivars could be detected quickly and efficiently using specific primer pairs and
restriction enzymes. The phylogenetic relationships of the 15 strawberry cultivars was separated
between the Festival (Fragaria x ananassa) cultivar group and the Californica (Fragaria x vesca)
cultivar group at a percent similarity of 48.352%.
Keywords: CAPS, cultivars,Fragaria spp., polymorphism, similarity index
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