| Abstrak/Abstract |
Strawberry (Fragaria spp.) is a fruit that has a high economic value and various benefits, but the production of strawberry plants in Indonesia is still low both in terms of quantity and quality. Strawberry plant breeding can be done in various techniques, one of which is polyploidization. Some previous studies have conducted polyploidization by an induction of colchicine in various concentrations in strawberry crops derived from Californica and Festival cultivars grown in the Village Banyuroto, Sawangan sub- district, Magelang district. The results of these studies indicate that colchicine could induce the strawberry plant having superior phenotype characteristics. Therefore, to prove the existence of a change in ploidy in strawberry crops as results of colchicine induction, then research at the molecular level needs to be done. The purposes of this study were to find out changes in ploidy of strawberry plants as results of colchicine induction using CAPS molecular markers, to determine the success of the CAPS molecular marker in detecting the number of ploidy quickly in strawberry (Fragaria spp.) plants, and determine the specific bands shown by strawberry plants having undergone polyploidization. The research was conducted from December 2014 to May 2015. The samples used were young leaves of strawberries namely, the leaves of Californica and Festival cultivars-derived strawberries having the best phenotype as results of colchicine induction. There were three procedures performed here, including the isolation of genomic DNA from leaves of the strawberry plant, DNA amplification, and the cutting of the resulting amplified DNA using restriction enzymes. The results from the analysis of ploidy with CAPS molecular markers showed that the Californica and Festival cultivar-derived strawberry (Fragaria spp.) plants treated with colchicine in previous studies have no experienced the addition of ploidy. CAPS molecular markers using specific primer pairs and the restriction enzyme are capable of detecting ploidy quickly and efficiently in strawberry plants. Specific bands among strawberry plants having undergone polyploidization and those not undergone polyploidization can be seen on the differences in monomorphic or polymorphic bands between the control plants and treatment plants. |
