| Abstrak/Abstract |
Staphylococcus aureus enterotoxins are essential in human and animal infection and food poisoning. This study analyzed ten genes (sek, sel, sem, sen, seo, sep, seq, ser, ses, set) encoding new staphylococcal enterotoxin (SE) isolated from animals and humans by multiplex polymerase chain reaction (PCR). A total of 183 Staphylococcus aureus isolates were obtained from goat samples (5 isolates), cattle samples (9 isolates), and samples from human infection cases (174 isolates) in Yogyakarta, Indonesia. All isolates were confirmed to be Staphylococcus aureus based on bacterial culture and biochemistry as well as identification of 23S rRNA. The sel gene was most often observed in Staphylococcus aureus isolates from goats (4 isolates, 80%), followed by the 20% sek gene. Isolates Staphylococcus aureus from cattle, the sel gene was most often found 44% (4 isolates), followed by sek gene 22%, sep gene 22% and ser gene 11%. Staphylococcus aureus isolates from humans were found with the most sem genes 22% (38 isolates), followed by 21% sel genes, 14% sek genes, 13% sep genes, 8% sen genes, and 2% ser genes. The sel gene was most often found in Staphylococcus aureus isolates from goats and cattle, while Staphylococcus aureus isolates from humans were found in the sem gene. Detecting a new type of SE among animals and humans indicates a public health threat due to SE infection. The occurrence of this new type of SE might be used as an approach for controlling infections and food poisoning diseases. |
