Detection of DNA Damage in Frozen Bovine Semen Using Eosin Staining
Penulis/Author
TEGUH ARI PRABOWO (1); Dr. Ir. Sigit Bintara, M.Si., IPU., ASEAN Eng. (2); Prof. Dr. Ir. Lies Mira Yusiati, SU., IPU., ASEAN Eng (3); Prof. Ir. Diah Tri Widayati, MP., Ph.D., IPM (4)
Tanggal/Date
2022
Kata Kunci/Keyword
Abstrak/Abstract
Background and Objective: Early detection of DNA defects in spermatozoa is vital because genetic abnormalities are associated with infertility and miscarriage. Eosin is an acidic stain with negatively charged chromatolytic components also found in the cytoplasm of spermatozoa. This study aimed to observe the effectiveness of eosin staining in detecting spermatozoa DNA deterioration.
Materials and Methods: A total of four-hundred frozen beef straws from Simmental, Limousin and Brahman cattle and Ongole crossbreeds, were obtained from the Singosari National Artificial Insemination Center, Malang, Indonesia. Hydrogen peroxide was used to degrade frozen sperm. In addition, the sperm was subjected to three treatments: Agarose trapping, membrane lysis and eosin staining.
Results: Damaged bull spermatozoa stained with eosin were bright red in color around the head and mid piece, whereas the head of undamaged spermatozoa were darker red. The average value of damaged DNA was 89.08±3.27% as assessed with eosin staining.
Conclusion: Eosin staining effectively detects DNA damage in bull spermatozoa, but extended effectiveness tests are considered
necessary
Rumpun Ilmu
Ilmu Peternakan
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Teguh Ari Prabowo_Detection of DNA Damage_Pak_ J_ Biol_ Sci_, 25 (5) 396-400, 2022.pdf
[PAK] Full Dokumen
2
Cek similarity.pdf
[PAK] Cek Similarity
3
02 Surat Keterangan Publikasi Detection of DNA Damage.pdf
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
4
PBJS-Diah- Teguh_230411_100506.pdf
[PAK] Bukti Korespondensi Penulis
5
Rev_Full dokumen Detection of DNA.pdf
[PAK] Full Dokumen
6
Rev_Cek similarity.pdf
[PAK] Cek Similarity
7
Rev_Rev_Cek similarity Detection of DNA Damage.pdf
