| Abstrak/Abstract |
This study aimed to analyze the bioactive constituents of chloroform (CHCl3) and ethanol (EtOH) extracts from marine sponges (Stylotella sp., Agelas dispar, Neopetrosia sp., Aaptos sp., Haliclona sp.) using TLC-bioautography technique, inhibitor tyrosinase activity, and anti-toxicity against B16F10 melanoma and Vero cells line. TLC studies used various solvent systems as mobile phases to identify active antioxidant agents. The inhibitory activity of tyrosinase was assessed using a colorimetric technique, and MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole] was used to analyze the viability of B16F10 and Vero cells treated with various concentrations (15.63-1000 μg/mL) of marine sponge extracts. TLC bioautography analysis utilizing different polarity mobile phases separated different bands from tested marine sponge CHCl3 and EtOH extracts with antioxidant activity. Polar substances in CHCl3 and EtOH extracts of marine sponges were found to significantly contribute to their antioxidant activity. The data showed that marine sponge Neopetrosia sp. and Aaptos sp. could reduce tyrosinase activity, and extracts at concentrations of 15.63-1000 μg/mL did not show substantial toxicity against B16F10 and Vero cells. Complex substances were proposed to be responsible for the antioxidant and tyrosinase inhibitor activity of Neopetrosia sp. and Aaptos sp. extracts. Data shows that marine sponges Neopetrosia sp. and Aaptos sp. might contain interesting antioxidants and tyrosinase inhibitors. |
